Composite

Part:BBa_K2906000:Design

Designed by: Elisa Barrow, Camilo Albornoz   Group: iGEM19_Manchester   (2019-10-14)


sfGFP with Tet Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 92


Design Notes

Type IIs compatible Codon optimised residue 6 from GAG to GAA (Glu)


Source

Superfolder GFP also known as sfGFP is a GFP-derived green fluorescent protein. (Pédelacq et al., 2006). GFP is a protein isolated from the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light in the blue to the ultraviolet range

References